【生物】enzyme immobilisation

[chocdumpling 10]

請問為什麼immobilised eznyme have a greater thermostability than non- immobilised enzyme???

for example when lactase is capsuled into sodium alginate beads, it has a greater thermostability than free lactase.

[Zeta 10]

酵素固定化

固定化的酵素比非固定化的酵素具有較高的熱穩定性,這是當然的了

酵素固定上去後,兩者之間產生化學鍵結,化學鍵的鍵能通常都比較高,要較高的能量才能打斷

至於這個,看它是化學吸附還是物理吸附來決定

化學吸附通常都會產生鍵結,物理吸附則否,只是附著在表面,而吸著(sorption)又介於化學跟物理吸附之間

我是學這方面的,但是我不懂生化,我學的是將surfactant固定在membrane上

重點是擺在hydrophobic以及hydrophilic上面

熱穩定性的關鍵就在兩者之間的interaction

可以去找一下資料

1.Principles of adsorption and adsorption processes

2.膜物理化學

固定化不是"Immobilization"嗎?........很少看到有人把z改成s的

[chocdumpling 10]

固定化不是"Immobilization"嗎?........很少看到有人把z改成s的

Immobilization 是美式英文耶.......

當然要講immobilisation才是正統的.....:p

i know that when lactase is immobilised it has a greater thermostability, but i need a detailed explaination . (of this specified case)

this is what i think,

--->Entrapment within a 3-D gel matrix is a common method of enzyme immobilisation, in this case lactase is held within an alginate capsule, cross linked with linear chains of Ca2+ ions. Thermal stability upon immobilisation is the result of molecular rigidity and the creation of a protected microenvironment. Therefore immobilised lactase has a higher denaturation temperature than free lactase.

But can this be further explained???

[Zeta 10]

Immobilization 是美式英文耶.......

當然要講immobilisation才是正統的.....:p

i know that when lactase is immobilised it has a greater thermostability, but i need a detailed explaination . (of this specified case)

this is what i think,

--->Entrapment within a 3-D gel matrix is a common method of enzyme immobilisation, in this case lactase is held within an alginate capsule, cross linked with linear chains of Ca2+ ions. Thermal stability upon immobilisation is the result of molecular rigidity and the creation of a protected microenvironment. Therefore immobilised lactase has a higher denaturation temperature than free lactase.

But can this be further explained???

不好意思,我沒有辦法針對這個特定的問題回答

這牽扯到生化的失活(denature),高分子交聯(cross-link)及分子剛硬度(rigidity),這些我都忘光了= ="" = =""

而且生物化學(biochemistry)我沒有唸過,高分子我太久沒碰了,現在想不起來了